Other Experimental Options for Evaluating Drug Abuse PotentialEx Vivo Binding and Microdialysis


RenaSci offers a battery of other in vitro, ex vivo and in vivo techniques that can be used to investigate the pharmacological mode of action of a drug as this can give an indication of its relative risk of being abused :-

     •     Ex vivo binding to determine compound occupancy of abuse-related receptors,
           binding sites and transporters  
     •     Intracerebral microdialysis in freely-moving rats to assess the abuse potential
           of compounds by their effects on extracellular levels of key neurotransmitters
           eg dopamine or 5-HT and their metabolites
     •     Correlation of drug levels with neurochemistry and behaviour using the
           Culex Bambino/Raturn system (plus drug levels in microdialysates if required)
     •     Neurochemical Fingerprinting - neurochemical phenotyping of substances of abuse
     •     Three Component Test – sequential determination of the effects of drugs on limbic 
           and striatal dopaminergic function (locomotor activity and stereotyped
           behaviour) and brain neurochemistry (dopamine and metabolites) in mice 
     •     Behavioural phenotyping – all types of substances of abuse
     •     Measurement of locomotor activity - stimulants and sedatives
     •     Repeated drug administration to assess the development of drug-induced
           sensitisation (eg hyperactivity or stereotyped behaviour)
     •     Head twitches -  a 5-HT2A  receptor-mediated behaviour in mice that can be used to
           detect potential hallucinogens

Ex Vivo Binding to Determine Compound Occupancy of Abuse-related Transporters
For further information about any of these supplementary tests to evaluate the abuse liability of novel compounds  please get in touch.