Intracerebral Microdialysis
Intracerebral microdialysis in freely-moving animals is a powerful tool that provides insights into the mode of action and safety (drug abuse liability) of centrally-acting drugs. RenaSci has over 10 years of experience of using microdialysis to support the development of drugs to treat a range of psychiatric, neurological and metabolic (obesity) disorders. Our capabilities in intracerebral microdialysis include :-
Mouse Microdialysis
Mouse microdialysis (single probe) can be performed in various brain regions including the striatum and hippocampus. Microdialysis can be conducted in normal or genetically-modified mice. Mouse microdialysis can therefore be used to provide vital information about the pharmacological mechanisms required to rectify abnormal brain neurotransmission in transgenic mice used to model specific CNS disorders.
Key features of mouse microdialysis include :-
- Drugs can be given po, ip, sc or by reverse dialysis
- Normal or genetically-modified mice
- Various brain regions
- HPLC with ECD optimised for each neurotransmitter
- State of the art ALEXYS™ monoamine analysis systems
- Monoamines and metabolites (simultaneously), GABA, glutamate, acetylcholine
- Optional blood and tissue-sampling in parallel groups of mice for PK analysis
- Smaller quantities of drug required than for rat microdialysis
Please contact us to find out more about mouse microdialysis.
Rat Microdialysis
Microdialysis can be performed in normal rats and in strains of animals modelling specific diseases such as spontaneously hypertensive rats to model attention deficit hyperactivity disorder. Drugs can be given by a variety of routes to freely-moving animals and extracellular levels of a variety of neurotransmitters (eg monoamines and their metabolites, acetylcholine, glutamate and GABA) can be determined in different brain regions by HPLC with electrochemical detection (ALEXYS™). Dual probe analysis can be used to measure the free concentration of different neurotransmitters in different brain regions simultaneously.
