RenaSci

Simultaneous Microdialysis, Blood-sampling and Behaviour

Simultaneous Microdialysis, Blood-sampling and Behaviour

 

We employ the Culex Bambino (BASi) for independent programmable collection of brain dialysate and stress-free blood sampling from freely-moving rats. These measurements can be coupled with behavioural monitoring (horizontal locomotor activity) using the Raturn (BASi). 

This elegant technique allows the simultaneous collection of pharmacodynamic and pharmacokinetic data, eg for the correlation of plasma levels of a drug with brain neurochemistry and/or behaviour. The Culex Bambino and Raturn can therefore be used to examine the mode of action and/or abuse liability of centrally-acting drugs.


Key features of this service include :-

•     Normal or genetically modified rats
•     Single or dual-probe studies in freely moving rats
•     Compounds dosed po, ip, sc or by localised reverse-dialysis (iv if only
      microdialysis and behavioural measurements are required)   
•     Various brain regions
•     HPLC with ECD optimised for each neurotransmitter or neuromodulator
•     State of the art ALEXYSTM analysis systems for measurement of
    - monoamines and their metabolites (simultaneously)
    - glutamate and GABA
    
       -  acetylcholine (with the inclusion of cholinesterase inhibitors)
•     Automated blood-sampling from the same rats to provide pharmacokinetic data                 on plasma drug levels
•     Behavioural monitoring (horizontal locomotor activity)

Culex Bambino

Simultaneous Measurement of Dopamine (Nucleus Accumbens) and Locomotor Activity Levels  

All studies are customised to meet the specific requirements of each client and include advice on experimental design, statistical analysis by a qualified statistician, fully audited data pack and written reports to regulatory standards if required.


Please get in touch for further information about simultaneous microdialysis, blood-sampling and behavioural measurements in the same animals. 
 
 

Posters

 

Heal et al. 2008. Demonstration of the relationship between the in vivo dopamine efflux, behaviour and drug pharmacokinetics. Presented at the College on Problems of Drug Dependence Meeting, Washington, DC, USA, 27th-28th October 2008.

 

Jackson et al. 2011. Comparison of the effects of equivalent doses of lisdexamfetamine dimesylate and d-amphetamine on extracellular concentrations of striatal dopamine, locomotor activity and plasma d-amphetamine concentrations in freely-moving rats. Program No 775.23. Society for Neuroscience Meeting, Washington, DC, USA, 12th-16th November 2011.

 

Rowley et al. 2011. Lisdexamfetamine dimesylate versus d-amphetamine - Hysteresis analyses of the relationships between extracellular striatal dopamine and locomotor activity measured in freely-moving rats. Program No 775.11. Society for Neuroscience Meeting, Washington, DC, USA, 12th-16th November 2011.

 

Rowley et al. 2012a. A microdialysis and behavioural comparison of lisdexamfetamine and methylphenidate in freely-moving rats. Presented at the NCDEU Meeting, Arizona Biltmore Resort & Spa,Phoeniz, Arizona, USA, 29th May -1st June, 2012.

 

Rowley et al. 2012b. A microdialysis and behavioural comparison of lisdexamfetamine, methylphenidate and modafinal in freely-moving rats. British Association for Psychopharmacology Summer Meeting, Harrogate, UK, 22nd-25th July 2012. Abstract No TI03. J. Psychopharm. Vol 26, Issue 8, August 2012.

 

Rowley et al. 2013. A microdialysis and behavioural investigation of modafinil in freely-moving rats. Abstract No. 547. Presented at the 75th Annual Scientific Meeting of the College on Problems of Drug Dependence, San Diego, California, USA, 15th-20th June 2013.

 

Manuscripts

 

Rowley et al. 2012. Lisdexamfetamine and immediate release d-amfetamine - Differences in pharmacokinetic/pharmacodynamic relationships revealed by striatal microdialysis in freely-moving rats with simultaneous determination of plasma drug concentrations and locomotor activity. Neuropharmacology 63(6): 1064-1074. [PubMed]

 

Rowley et al. 2014. Differences in the neurochemical and behavioural profiles of lisdexamfetamine, methylphenidate and modafinil revealed by simultaneous dual-probe microdialysis and locomotor activity measurements in freely-moving rats. J Psychopharmacol 28(3): 254-269. [PubMed]