RenaSci

Immunohistochemistry and Histology Services

Immunohistochemistry and Histology

RenaSci offers a range of immunohistochemical and histological techniques which can be used to examine changes in the CNS at the cellular level following acute or chronic drug treatment. These techniques can be used to investigate the mode of action or potential neurotoxicity of project compounds. Our immunohistochemistry and histology services can be used for specific studies or as a cost effective add-on service during the early phase of drug discovery. 

RenaSci can perform as much or as little of the processing and analysis as you require from tissue preservation through to quantification by image analysis.

The team has expertise in microdissection of various brain regions in rats and mice, including the frontal cortex, striatum, hypothalamus, hippocampus and cerebellum.  Tissue samples can be taken and frozen by a variety of methods, for example, using isopentane, liquid nitrogen or dry-ice or can be formalin-fixed and wax embedded to produce a tissue block.

RenaSci has the capabilities to section wax-embedded tissue using a microtome (≥ 4 µM thick); frozen tissue using a cryostat (15-25 µM thick) or fresh or fixed tissue using a vibrotome (≥ 200 µM thick).


We can perform immunohistochemical or histological analysis for you in-house or alternatively ship tissue sections (mounted onto microscope slides) or formalin-fixed or wax-embedded tissue blocks to laboratories of your choice. 

We can also conduct immunohistochemical and histological analysis on tissues provided from studies performed at other facilities. 

Immunohistochemical services currently on offer at RenaSci include :-

•    5 HT (serotonin)
•    Tyrosine hydroxylase
•    Tryptophan hydroxylase
•    Neurofilament
•    Microtubule Associated Protein-2 (MAP-2)
•    c-Fos
•    Glial Fibrilliary Acidic Protein (GFAP). 

Immunohistochemistry and Histology

Our range of histological stains include :-

•    Haematoxylin and Eosin staining for routine staining of cell structures
•    Luxol fast blue for staining of myelin in the CNS and spinal cord
•    Cresyl violet (Nissl) to detect neuronal cell bodies in the CNS and spinal cord

Stains for assessment of neuronal cell death include :-

•   Propidium iodide for assessment of necrosis
•   TUNEL staining for assessment of apoptosis

Light and fluorescence digital images can be captured and quantitative image analysis performed using Leica QWinPro image analysis software to count cell number, size and percentage area of staining which can be used as a marker of neuronal sprouting or degeneration. 

In addition to the examples given above, we have the skills and expertise to develop protocols and optimise assay conditions using any commercially available antibodies and stains.


Please get in touch for further information about our immunohistochemical and histology services.