RenaSci

Mouse Microdialysis

HPLC

 

RenaSci has established and validated the powerful tool of intracerebral microdialysis in freely-moving mice. We are world leaders in mouse microdialysis and one of a very small number of laboratories able to perform this sophisticated technique. Mouse microdialysis can be used to measure extracellular levels of the main neurotransmitters and neuromodulators in various brain regions including the striatum and hippocampus. Microdialysis in mice can therefore be employed by the pharmaceutical and biotech industry to assess the mode of action and/or drug abuse liability potential of novel centrally-acting compounds.


Microdialysis also provides vital information to help us understand brain malfunction and the pharmacological mechanisms required to rectify abnormal brain neurotransmission in transgenic mice used to model specific CNS disorders.

Microdialysis in Freely-moving Mice

 

Key features of our mouse microdialysis service include :-

 

     •   Normal or transgenic mice
     •   Compounds dosed po, ip, sc or by reverse-dialysis
     •   Small quantities of drug required
     •   Single probe studies (dual probe available for monoamines in certain brain regions)
     •   HPLC with ECD optimised for each neurotransmitter or neuromodulator
     •   State-of-the art ALEXYS™ analysis systems for measurement of monoamines
         and their metabolites (simultaneously), glutamate and GABA
     •   Optional blood and tissue-sampling from parallel groups of mice to provide
         pharmacokinetic/pharmacodynamic data on brain neurotransmitter and drug levels
         and plasma drug levels

 

Neurotransmitters and metabolites can be detected down to the following levels :-


     •   Dopamine (40 pM); DOPAC (2 nM); HVA (2 nM)
     •   Noradrenaline (200 pM)
     •   5-HT or serotonin (100 pM); 5-HIAA (2 nM).
     •   Glutamate (8 nM)
     •   GABA (6 nM)

 

Verification of probe placement into mouse striatum (brain fixed in buffered formalin, embedded in wax and sectioned prior to staining with Haematoxylin and Eosin).

 Microdialysis Probe Placement

Please contact us for further information about mouse microdialysis.


Posters


Heal et al. 2010. Further validation of “neurochemical fingerprinting” to characterise drugs with different presynaptic dopaminergic mechanisms: comparison with in vivo microdialysis. Program No. 669.22. Society for Neuroscience, San Diego, USA, 2010.