RenaSci

Three Component Test of Dopaminergic FunctionThree Component Test

 

The three component test is a rapid and efficient technique that can be used to profile the effects of drugs on limbic and striatal dopaminergic function and neurochemistry in the same mice.

 
The three components of the test (measured as part of a single experiment) are :-


     •     Locomotor activity (a mesolimbic mediated behaviour)
     •     Stereotyped  licking, biting and chewing (extrapyramidal-mediated behaviours)
     •     Measurement of striatal levels of dopamine and its metabolites (neurochemical
           fingerprinting)

 

The three component test can be used to :-


     •     Identify novel antipsychotics (following administration of amphetamine which
           can be given in divided doses)
     •     Profile dopamine reuptake inhibitors, dopamine releasing agents and other dopamine
           modulators
     •     Demonstrate that drugs have little effect on the dopamine system in vivo.

 

Effect of Methylphenidate on Locomotor Activity and Stereotypy in Mice

Neurochemical Fingerprint of Methylphenidate in Mice 

Please contact us for further information about the three component test.

 

Posters

 

Cheetham et al. 2009. Sequential determination of limbic and striatal behavioural function and dopamine neurochemistry in C57/BL6 mice. Program No. 843.16. Society for Neuroscience Meeting, Chicago, Illinois, USA, 17th-21st October 2009.

 

Cheetham et al. 2010. Differences in presynaptic mechanisms of the ADHD drugs, d-amphetamine and methylphenidate, revealed by "neurochemical fingerprinting”: correlations between dopamine neurochemistry and behavioural function. Program No 669.21. Society for Neuroscience Meeting, San Diego, California, USA, 13th-17th November 2010.