Pair-feeding Studies

Pair-fed Studies


Pair-feeding studies in animals can be used to explore the contribution made by reduced food intake to drug-induced reductions in body weight. In these studies, the amount of drug eaten by the drug treatment group each day is determined and given to a vehicle-treated ‘pair-fed’  group of animals housed under identical conditions the following day. If the weight-loss in the pair-fed animals is the same as the drug-treatment group, the drug is likely to be reducing body weight by inhibiting food intake. Drugs acting on both sides of the energy balance equation (reducing food intake and increasing energy output) would be expected to produce greater weight-loss than the corresponding pair-fed group.

Key features of pair-feeding studies :-

     •     Mice or rats
     •     Normal, high fat or cafeteria diet
     •     Drugs can be given sub-chronically or chronically by a variety of routes
     •     Studies can examine the effects of pair-feeding on a variety of other 
           parameters eg glycaemic control and body composition analysis


Please contact us for further information about pair-feeding studies in rats or mice.



Vickers et al. 2008. Effect of rimonabant on body weight, glucose tolerance, body composition and lipolysis in fa/fa Zucker rats: A comparison with pair-fed controls. Program No. 584.18. Society for Neuroscience Meeting, Washington, DC, 15th-19th November 2008.




Gardiner et al. 2010. Prokineticin 2 is a hypothalamic neuropeptide that potently inhibits food intake. Diabetes 59: 397-406. [PubMed]