RenaSci

Inhibition of Gastric Emptying

Inhibition of Gastric Emptying

 

Inhibition of gut motility has been linked to satiety and reduced food intake therefore we have developed the phenol red recovery model in mice to evaluate the ability of drugs to inhibit gastric emptying.

 

Key features of the phenol red assay are :-

 

     •     Mice deprived of food for 22 h and water for 2 h before testing
     •     Vehicle and drugs given by a variety of routes
     •     Following a suitable pretreatment time (eg 60 min) mice dosed with vehicle or
           phenol red (0.05% w/v) po 
     •     Amount of phenol red in stomach determined immediately (control group,
           maximal 100% absorbance) or 30 min later (phenol red-treated mice)
     •     Absorbance assessed by spectrophotometry
     •     Results expressed as % gastric emptying

 

The model has been validated at RenaSci using the opioid agonist, loperamide, and the GLP-1 agonist, exendin-4  (exenatide). Both compounds significantly decreased % gastric emptying compared to control animals.

Inhibition of Gastric Emptying by Loperamide

Inhibition of Gastric Emptying by Exendin-4

 

We have also recently developed a solid-food model to investigate the ability of drugs to inhibit gastric emptying.

 

Please contact us for further information about our animal models to measure the effects of drugs on gut motility.