RenaSci

New Metabolic Profiling Service using the TSE PhenoMaster

 29/11/2018 01:54

RenaSci are now offering simultaneous and fully automated measurement of energy expenditure (resting metabolic rate, meal-induced thermogenesis and physical activity) and energy input (food intake) using the TSE PhenoMaster. Parameters measured by the 16-cage indirect calorimetry system include: 

   • Oxygen consumption (VO2)

   • Carbon dioxide production (VCO2)

   • Energy expenditure (derived from VO2 and VCO2)

   • Respiratory exchange ratio (RER; which estimates relative substrate utilization, ie shifts in fat and carbohydrate utilization)

   • Food and water intake

   • Meal and drink patterns (ie frequency and size)

   • Physical activity (measured as beam breaks on the x, y and z axis)


The PhenoMaster uses standard individually ventilated cages to resemble the home cage. Readings are provided for each cage every 20 min for up to 5 days. Data can be calculated over various time-points eg hourly, daily or to determine photoperiod-specific effects. Metabolic profiling can be performed as part of a chronic study or as a standalone acute study. 


We have validated the TSE PhenoMaster System by assessing the effects of the beta3-adrenergic receptor agonist, CL-316,243 on energy expenditure and energy input in dietary-induced obese mice.  CL-316,243 produced weight-loss, which was associated with increased energy expenditure, increased utilization of fat (reduced RER) and reduced food intake (reductions in both the number and size of each meal). The increased energy expenditure was independent of changes in physical activity. 


Click here to see the data.


These results demonstrate that metabolic profiling by the TSE PhenoMaster is a useful tool for gaining insight into the mode of action of novel antiobesity drugs. 


Please contact inform@renasci.co.uk for further information about our new metabolic phenotyping service.