Behavioural Profiling

We offer a wide range of behavioural and physiological assays that can be used to assess the mode of action, efficacy and side-effect profile of centrally acting drugs. They can also be used in proof-of-concept studies for novel molecular targets in the brain. These assays include:

  • Behavioural phenotyping (transgenic animals)
  • Initial safety assessment of novel compounds
    • Irwin test
    • Rotarod
  • Neurotransmitter-specific functional tests
  • Measurement of the effects of drugs on seizure threshold
  • Pupillometry

Effect of Diazepam in the rat rotarod test

Data are back-transformed adjusted means (n=12). SEMs are calculated from the residuals of the statistical model.
Data was analysed by ANOVA on square root transformed data averaged over trials 1-3 for each animal.
Comparisons to vehicle for Diazepam by Williams’ test ***p<0.001.

 

Drugs (alone or in combination with a receptor agonist or antagonist) can be given by a variety of different routes (including intracerebroventricular injection). Blood and tissue samples can be collected for pharmacokinetic or neurochemical analysis or ex vivo binding if required.

 

 

Pupillometry at RenaSci

 

Adding to our portfolio of CNS services, we have recently developed a model of Morphine- and Clonidine-induced mydriasis (see figures below).
These data demonstrate the temporal effects of these agents to dilate the pupils of conscious rats. This assay is being utilised to better understand the pharmacology of novel centrally acting drugs.

 

Clonidine-induced mydriasis in rats

 

Data are back-transformed adjusted means ± SEM (n=8).  Analysis was by two-way analysis of covariance
of log transformed data with treatment and day as factors and pupil diameter at 0 mins as a covariate.
Comparisons against vehicle were by Williams’ test, **p<0.01, ***p<0.001.

 

 

Morphine-induced mydriasis in rats

 

Data are back-transformed adjusted means ± SEM (n=8-16). Analysis was by one-way analysis of covariance
of log transformed data with treatment as a factor and pupil diameter at 0 mins as a covariate.
Comparisons against vehicle were by multiple t-test ***p<0.001.

We work with clients to develop and validate novel behavioural and physiological functional assays.

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