RenaSci offers a range of immunohistochemical and histological techniques. These can be used to examine changes in the CNS at the cellular level following both acute or chronic drug treatment. They can also be used to investigate the mode of action or potential neurotoxicity of project compounds.

c-Fos immunohistochemistry

Our immunohistochemistry and histology services can be used for specific studies, or as a cost-effective add-on service during early-phase drug discovery.

• Microdissection of various brain regions in rats and mice
• RenaSci can conduct as much or as little of the processing and analysis as required
• Tissue preservation
  • Frozen – liquid nitrogen/dry ice/isopentane
  • Formalin-fixed
  • Tissue embedding to wax block
• Tissue sectioning
  • Microtome/Cryostat/Vibrotome
• Immunohistochemical stains
  • 5-HT (serotonin)
  • Tyrosine hydroxylase
  • Tryptophan hydroxylase
  • Neurofilament
  • MAP-2
  • c-Fos
  • GFAP
• Histological stains
  • Haematoxylin and Eosin
  • Luxol fast blue
  • Cresyl violet (Nissl)
• Stains for assessing neuronal cell death
  • Propidium iodide
  • TUNEL
• Quantitative image analysis.

Analyses can be performed on samples from external sources.

We have the skills and expertise to develop protocols and optimise assay conditions using any commercially available antibodies and stains.