Microdialysis

Microdialysis

RenaSci is a leading provider of intracerebral microdialysis services in rats and mice. We have been conducting microdialysis for more than 20 years. Our dedicated team of experts can design bespoke microdialysis studies to meet clients’ precise needs.

Microdialysis can be used to study the effects of drugs on free concentrations of neurotransmitters and neuromodulators in different brain regions of freely moving or anaesthetised rodents.

Microdialysis probes

 

Our experts use high sensitivity UHPLC and HPLC with electrochemical detection (ALEXYS™) to measure:

 

  • Dopamine
  • Noradrenaline
  • Serotonin
  • Monoamine metabolites
  • Gamma-amino butyric-acid (GABA)
  • Glutamate
  • Acetylcholine
  • Others (please contact us for further details)

 

Dual-probe microdialysis can be used to measure the effects of drugs on multiple neurotransmitters and their metabolites in the same study.

Compounds can be given by a variety of routes, including localised reverse dialysis. Samples can be taken for up to 24 h following drug administration.

One or two examples of data only (methylphenidate figures as already in correct format or cocaine flyers or lisdexamfetamine figures but would need to put in correct format).

Dual probe microdialysis:

  1. Dopamine, striatum

Dual Probe Microdialysis- Dopamine, Striatum

    2. Noradrenaline, frontal cortex

Noradrenaline, Frontal Cortex

Dopamine, DOPAC, HVA

Dopamine, DOPAC, HVA

 

Rat microdialysis can be combined with behavioural assessment and automated blood sampling (using the Culex Bambino/Raturn System; BASi) for PK measurements to generate a detailed PK/PD assessment, or blood and tissue samples can be taken from parallel groups of rats.

This can also be used to look at the recovery and measurement of free drug concentrations in the brain, or to measure drug and neurotransmitter levels in the same microdialysate sample.

Contact us

For more details or information on how we can support your project please complete the contact form.