The H-FFC Cl₄ mouse develops severe NASH with bridging fibrosis within 12 weeks

 

• Mice are maintained on a diet high in fat, fructose and cholesterol (H-FFC) and dosed with CCl₄ for 12 weeks
• The H-FFC CCl₄ mouse develops all the clinical histological endpoints associated with NASH including bridging fibrosis
• Liver bioanalysis confirms the histological findings
• Plasma AST is significantly increased

 

The H-FFC CCl₄ mouse develops severe NASH and bridging fibrosis

 

Histopathology scoring of standard chow and H-FFC CCl₄ mice. Data are presented as individual animal scores and means ± SEM.
Significant differences versus the standard chow vehicle group are denoted by ***p<0.001. The NAFLD activity score (NAS) was calculated by combining the scores for # parameters.

 

 

 

Sirius red stained liver – demonstrating the presence of bridging fibrosis

 

 

Histological endpoints are confirmed with liver bioanalysis, indicated by a significant increase in collagen, triglyceride and cholesterol content in the H-FFC CCl₄. Plasma levels of the liver enzyme ALT is also significantly increased.

 

 

 

Liver biochemistry and terminal plasma analysis of standard chow and H-FFC CCl₄ mice.
Data are expressed as the means and SEM. Significant differences versus the standard chow group are denoted by **p<0.01 and ***p<0.001.

 

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