Body composition analysis can be used to investigate whether potential anti-obesity drugs reduce body weight by producing a clinically beneficial selective loss of fat, or by producing non-specific decreases in water (dehydration) and/or protein content (muscle wasting or cachexia), which can be harmful.
Body composition analysis can be determined by classical chemical analysis, by a FoodScan™ near-infra-red meat analyser, or by dual-energy X-ray absorptiometry (DEXA).
Key features of the gold-standard chemical analysis method are:
The reduction in body weight produced by the MCH1 antagonist,
GW803430, in DIO mice is due to selective fat loss
Chemical analysis can also be used to determine faecal fat, protein, water and ash content and the composition of individual tissues, e.g. total liver fat content.
The FoodScanTM analyser has AOAC (Association of Official Analytical Chemists) approval as a reference method for the analysis of moisture, fat and protein in meat and meat products.
Key features of body composition analysis performed using the FoodScan™ analyser are:
RenaSci can provide bone and tissue composition analysis in mice using a Lunar PIXImus Densitomer. We have validated the DEXA technology using classical chemical extraction techniques.
Measures provided by DEXA include:
DEXA is quicker and cheaper than conventional body composition analysis and can be performed in live animals to provide longitudinal measurement of bone and tissue composition throughout chronic mouse studies.
Longitudinal DEXA measurement of body composition in
DIO mice given rimonabant or switched to a low fat diet
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