Chronic Feeding Studies

Chronic Feeding Studies

Experimental > Obesity > Chronic Feeding Studies

Chronic feeding studies are required to evaluate the effects of novel anti-obesity drugs on bodyweight and the intake of food and water.

The chronic feeding studies can be performed in normal, lean rats or mice, or in animal models of obesity. Drugs can be given by a variety of routes (including sustained infusion via sc implanted osmotic minipumps or programmable, refillable micro-infusion iPRECIO® pumps).

Bodyweight and intake of food are measured routinely in chronic feeding studies.

Other endpoints in chronic feeding studies include:

  • Glucose tolerance tests (to measure glycaemic control)
  • Blood-sampling to measure plasma levels of metabolic parameters and biomarkers
  • Blood and tissue-sampling for pharmacokinetic analysis
  • Blood pressure
  • Urinary glucose excretion
  • Faecal fat content
  • Pancreatic insulin levels
  • Liver lipids
  • Body composition analysis
  • Ex vivobinding to confirm brain penetration/site of action
  • Necropsy and gross post-mortem analysis
  • Preparation of tissues for histology and immunohistochemical (IHC) and histological staining

 

Effect of Semaglutide on body weight in dietary-induced obese mice

 

body weight in dietary-induced obese mice

n = 10. Significant differences against vehicle are denoted by ***p<0.001. Arrows indicate dosing days.

 

 

Effect of Semaglutide on cumulative daily food intake in dietary-induced obese mice

Effect of Semaglutide on cumulative daily food intake in dietary-induced obese mice

 

n = 10. Significant differences against vehicle are denoted by ***p<0.001.

 

 

Effect of Semaglutide on body composition (DEXA scan) in dietary-induced obese mice

 

body composition (DEXA scan) in dietary-induced obese mice

n=10. Significant differences against vehicle are denoted by **p<0.01, ***p<0.001.

 

 

Effect of Semaglutide on liver lipids in dietary-induced obese mice

 

liver lipids in dietary-induced obese mice

n=10. Significant differences against vehicle are denoted by *p<0.05, ***p<0.001.

 

 

 

Effect of Semaglutide on glucose tolerance in dietary-induced obese mice

 

glucose tolerance in dietary-induced obese mice

n=10. Significant differences against vehicle are denoted by *p<0.05, ***p<0.001.

 

 

Effect of Semaglutide on the plasma parameters in dietary-induced obese mice

 

plasma parameters in dietary-induced obese mice

n=10. Significant differences against vehicle are denoted by ***p<0.001.

 

 

 

If you are interested in any of our obesity or chronic feeding studies, please get in touch to find out more.

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