Inhibition of Gastric Emptying

The inhibition of gastric emptying has been linked to satiety and reduced food intake. Our experts at RenaSci, therefore developed the phenol red recovery model in mice. This allows the ability of drugs to inhibit gastric emptying to be evaluated.

 

The key features of the phenol red assay are:

 

  • Mice deprived of food for 22 h and water for 2 h before testing
  • Vehicle and drugs given by a variety of routes
  • Following a suitable pretreatment time (eg 60 min) mice dosed with vehicle or phenol red (0.05% w/v) po
  • Amount of phenol red in stomach determined immediately (control group, maximal 100% absorbance) or 30 min later (phenol red-treated mice)
  • Absorbance assessed by spectrophotometry .

 

The model has been validated using the opioid agonist loperamide, and the GLP-1 agonist Exendin-4 (exenatide). Both compounds decreased the percentage of gastric emptying compared to control animals.

 

 

Inhibition of gastric emptying in mice
by the opioid agonist, loperamide

 

Inhibition of gastric emptying in mice by the opioid agonist, loperamide

 

Results are expressed as adjusted means + SEM (n=6). Statistical analysis was by one-way analysis of variance. Loperamide was compared to vehicle by Williams’ test and significances are denoted by ***p<0.001.

 

 

Inhibition of gastric emptying in mice
by the GLP-1 agonist, exendin-4

 

Inhibition of gastric emptying in mice by the GLP-1 agonist, exendin-4

 

Results are expressed as adjusted means + SEM (n=6). Statistical analysis was by robust regression model with treatment as a factor. Exendin-4 was compared to vehicle by Williams’ test and significances are denoted by **p<0.01, ***p<0.001.

 

We also provide a solid food model to investigate the ability of drugs to inhibit gastric emptying.

Please contact us to learn more about our animal models for measuring gut motility.

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