Inhibition of Gastric Emptying

Inhibition of gut motility has been linked to satiety and reduced food intake. We therefore developed the phenol red recovery model in mice. This allows the ability of drugs to inhibit gastric emptying to be evaluated.

Key features of the phenol red assay are:

  • Mice deprived of food for 22 h and water for 2 h before testing
    •     Vehicle and drugs given by a variety of routes
    •     Following a suitable pretreatment time (eg 60 min) mice dosed with vehicle or phenol red (0.05% w/v) po
    •     Amount of phenol red in stomach determined immediately (control group, maximal 100% absorbance) or 30 min later (phenol red-treated mice)
    •     Absorbance assessed by spectrophotometry .

The model has been validated using the opioid agonist loperamide, and the GLP-1 agonist Exendin-4 (exenatide). Both compounds decreased the percentage of gastric emptying compared to control animals.

 

Inhibition of gastric emptying in mice
by the
opioid agonist, loperamide

Inhibition of gastric emptying in mice by the opioid agonist, loperamide

 

Inhibition of gastric emptying in mice
by the GLP-1 agonist, exendin-4

Inhibition of gastric emptying in mice by the GLP-1 agonist, exendin-4

We also provide a solid food model to investigate the ability of drugs to inhibit gastric emptying.

Please contact us to learn more about our animal models for measuring gut motility.

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