Lipolysis

We have developed and validated an assay for measuring lipolysis directly using isolated adipose tissue explants from mice or rats. Both stimulatory and inhibitory effects of drugs on lipolysis can be detected.

The assay for measuring lipolysis can be used to determine the acute effects of drugs on basal and isoproterenol-stimulated lipolysis in vitro, or basal and isoproterenol-stimulated lipolysis ex vivo, in tissues from animals following acute or chronic drug treatment. Adipose tissue can be taken from lean animals, or from any of our animal models of obesity and diabetes.

In a typical experiment, adipose tissue explants are incubated for 3h in the appropriate treatment. Glycerol released into the culture media is measured as an indicator of lipolysis. Other markers secreted from the adipose tissue into the media can also be measured (eg NEFA).

Isoproterenol-stimulated lipolysis: Lean and DIO mice

 

Measuring Lipolysis Assay

n = 6 experiments, epididymal tissue **p<0.01, ***p<0.001 vs control. 

 

Insulin-inhibition of isoproterenol-stimulated lipolysis: Mice on high fat diet for 1-2 or 8-9 weeks

 

n = 4 experiments, epididymal tissue *p<0.05, **p<0.01, ***p<0.001 vs control. 

 

 

Please contact us to find out more about our measuring lipolysis assays.

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